Glycosylation causes an apparent block in translation of immunoglobulin heavy chain.

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Glycosylation causes an apparent block in translation of immunoglobulin heavy chain.

Analysis of nascent heavy chains isolated from MPC11 (gamma 2b heavy chains) and MOPC 21 (gamma 1 heavy chains) mouse myeloma cells demonstrates an accumulation of nascent heavy chains which are slightly smaller in mass (approximately 35,000 daltons) than nascent heavy chains which have just been glycosylated (approximately 38,000 daltons). The accumulation of 35,000-dalton nascent heavy chain ...

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Two variants in immunoglobulin heavy chain production, derived from the MPC 11 mouse myeloma cell line, make short heavy (H) chains with identical precise deletions of the CH3 domain. The CH3 domain is expressed in the H chain mRNA from both variants. Although in vitro translation of this mRNA produces one H chain species, deleted heavy chains are secreted as heavy-light (HL) and H2L2 moieties ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1981

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)70031-3